Answer:
A) A transgene integrated into a heterochromatic region of the genome.
Explanation:
This is most likely the reason why the transgene is not expressed in the fifth cell line. It is likely that the transgene was integrated into a heterochromatic region of the genome. Heterochromatin consists of genetically inactive satellite sequences, and many of these are repressed to various extents. Moreover, because this is a tightly packed form of DNA or condensed DNA, it is inaccesible to polymerases and thus not transcribed.
Eukaryotic cells do not have a nucleus. The nucleus is where DNA, your genetic material, is usually stored. Prokaryotic cells do not have a nucleus. The DNA just floats around the cell instead of staying in one designated area.
From what I remember it's called a substrate
Maybe it's the concentration of water in your blood would increase (c)