Answer:
The correct answer is i forgot to label the probe with a reporter molecule.
Explanation:
Reporter molecule is a specific type of molecule that reports the success of cloning.
In this experiment to test the presence of N gonorrhoeae by using the DNA probe that was developed by me should be at first labelled probe sequence with a reporter gene such as lucciferase protein.
When this reporter molecule labelled DNA probe will bind to the target DNA sequence of N gonorrhoeae at that time by using the substrate specific for that reporter molecule will help me to notice the process of hybridization.
The cycle then repeats itself as precipitation falls
Answer:
it varies from country to country
Answer:
Agarose gel electrophoresis is the most suitable method for this analysis.
Explanation:
SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, is a procedure used for visualizing and separating proteins according to their molecular weight. The use of sodium dodecyl sulfate and polyacrylamide gel eliminates most of the influence of the tertiary structure and charge, and proteins are separated solely based on polypeptide chain length. So, if the student suspects that the protein sample is conformed of 4 subunits similar in molecular weight, SDS PAGE will likely not separate them.
On the other hand, an agarose gel electrophoresis can separate proteins based on their size AND charge, so, if you suspect that the proteins are not identical but similar in size, the charge factor can be important to separate them in an experiment.
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