To transform bacteria with plasmids, technicians first make the bacteria competent (capable of taking up DNA) by placing them in
calcium chloride and chilling them. Plasmid is then added to the competent bacteria and the plasmid/bacteria combination is taken through a few more steps to make the bacteria take up DNA. In your experiment, should you treat a tube of bacteria that you do not add plasmid to exactly as you do the tube of bacteria that you will transform:
The Plasmid is known as a genetic structures present in a cell that can be replicated independently of the chromosomes. Bacteria can take up from the foreign DNA in a process is known as the transformation.
This experiment helps to give control demonstrating that the arc (antibiotic-resistance colonies) which occurs on the plate is known as a result of the Plasmid which is taken up by the cells. If there is no Plasmid, then there should be no growth present on the antibiotic plates.
