Krebs cycle is a series of enzymatic reactions that catalyzes the aerobic metabolism of fuel molecules to carbon dioxide and water, thereby generating energy for the production of adenosine triphosphate (ATP) molecules.
Monosaccharides are the most basic form of sugar and can not be broken down into a smaller form. They are usually colorless, water-soluble, and crystalline solids. Examples of monosaccharides include glucose and fructose.
Answer:
In this ELISA procedure, a sample of protein is immobilized in the plastic well and then antibody for this protein added and incubated, and cleared out. In the given setting detection, the antibody is conjugated with the protein of interest and if the substrate molecule added to the reaction it will produce a colored product by reacting with the enzyme.
No reaction will take place in the well in case of not adding secondary antibody or detection antibody to the reaction and no colored product will be received.
