Answer:
Cut open the plasmid and "paste" in the gene. This process relies on restriction enzymes (which cut DNA) and DNA ligase (which joins DNA).
Insert the plasmid into bacteria. Use antibiotic selection to identify the bacteria that took up the plasmid.
Grow up lots of plasmid-carrying bacteria and use them as "factories" to make the protein. Harvest the protein from the bacteria and purify it.
Explanation:
I think the answer is D. A good indicator is the resting heart rate. Exercise strengthen your heart and it makes it more efficient. The resting heart rate shows of the heart is pumping blood normally each time it contracts. Having a normal resting heart rate would mean a healthy heart.
Based on these facts, we can state that the genes of the two organisms had a eukaryotic origin.
We can arrive at this answer because:
- Despite having exons, prokaryotes do not have introns in their genomes.
- This means that if two organisms have the same sequence of introns and exons, they have a common ancestor, in addition to having their genes originated in a eukaryotic organism, which explains the sequence of introns.
This means that the genetic expression of these two organisms has similarities, even if they are organisms with different biological processes.
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Microorganisms can be divided into two groups:
1. Archea
• Archea are prokaryotic unicellular organisms without cell nucleus or other membrane bound-organelles.
• Their membrane is built from ether lipids, which differs them from bacteria’s cell membrane.
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2. Bacteria
• Bacteria are also unicellular prokaryotic organisms.
• Bacterial cell membranes are made from phosphoglycerides with ester bonds. Bacterial genome is in a form of circular chromosome.
• They reproduce by binary fission or by budding.
Besides, prokaryotes (Archea and Bacteria) a large number of eukaryotes are also microorganisms. Unicellular eukaryotes contain organelles such as the cell nucleus, the Golgi apparatus and mitochondria. Usually, they reproduce asexually by mitosis. An example of eukaryotic microorganism is Protist.
