At the molecular level, the heat comes from the agitation of the molecules and therefore their atoms. This increase in agitation causes the molecules to be less condensed between them and will separate after breaks in the intermlecular bonds. This distends the material, as we see when we heat a metal, or a transformation of the phase of the material, such as melting ice or boiling water.
Answer:
It has incomparable advantages over other gene editing tools.
Explanation:
For example, the CRISPR-Cas9 system has more target sites than ZFNs and TALENs, and Cas9 has many variants that can be used in a variety of studies. Moreover, the system is extremely easy to use and can be executed in almost any laboratory.
The most important advantages of CRISPR/Cas9 over other genome editing technologies is its simplicity and efficiency. Since it can be applied directly in embryo, CRISPR/Cas9 reduces the time required to modify target genes compared to gene targeting technologies based on the use of embryonic stem (ES) cells.
Disadvantages of CRISPR technology: CRISPR-Cas9 off-target; The effect of off-target can alter the function of a gene and may result in genomic instability, hindering it prospective and application in clinical procedure.
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