D is your answer 
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 <span> A </span>test cross<span>, involves the breeding of an individual with a phenotypically recessive individual.
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Do you want the catholic answer or do you just want a general answer, because it could be an opinion.
        
                    
             
        
        
        
Explanation:
The out come that mendel's observed from dihybrid crosses confirmed that each trait could be described by a pair of factors that segregated to form progeny (his first law) and further suggested that factors for multiple traits segregated independently, thus forming the basis for mendel's second law of inheritance 
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Answer:
Recombinant plasmid
Explanation:
Researchers can insert DNA fragments or genes into a plasmid vector, creating a so-called recombinant plasmid. 
This plasmid can be introduced into a bacterium by way of the process called transformation.