Answer and Explanation:
The steps for construction of the expression plasma
- STEP 1 : Introducing Notl and Hindlll confinement sites at the ends of the gene of interest by means of PCR
- STEP 2 :Digest the plasmid and DNA encoding gene with the Notl and Hindol confinement endonucleuses.
- STEP 3 : Ligate the cut plasmid and DNA fragment
- STEP 4 : Change the plasmid into E.coli for expression
- STEP 5 : Clean the protein by utilizing a column of immobilized
umm the answer is c but you should show your work
Answer:
Protein synthesis is the process in which cells make proteins.It occurs in two stages: transcription and translation. Transcription is the transfer of genetic instructions in DNA to mRNA in the nucleus. After a polypeptide chain is synthesized, it may undergo additional processing to form the finished protein.
Explanation:
I hope this helps.
1. True. Establishing more sites for the protection of biodiversity across the world can go a long way and would have a chain reaction in the ecosystem. Thus, it will save a lot of our planet's biodiversity. 2. a. -Philiab. -trophicc. eco-d. eu-3. A habitat is where certain species, animals, or organisms naturally live, feed, reproduce, etc. Examples: ocean, rainforest, deserts. An ecosystem has two components: living and non-living organisms which interact with each other to maintain homeostasis. The primary difference between the two is the presence of interaction between the things in the environment.4. Energy5. Producers6. Sun7. (No choices provided) One of the top factors that threaten the survival of species is caused by humans, man-made phenomena. 8. Answer: Polar bears. Polar bears are endangered species and there are only approximately 20,000-30,000 polar bears left in the world. Their population had been decreasing due to habitat loss because of climate change. 9. Scientists based the background extinction rate on the number of known species that went extinct over a period of time in the past. They also based it on archaeological evidence of species that existed and went extinct years ago.
