Answer:
Measurement of peripheral blood cytokines and other immunomodulatory proteins is a useful and popular tool for assessing human immune responses to a wide range of assaults. A common challenge in this work is obtaining fresh, high-quality samples and limiting the time between blood collection and the separation of plasma or serum from cells. In this study we sought to determine the effect of sample age at the time of processing on the measured levels of 41 soluble immune mediators. Two cohorts were examined: healthy lab donors and trauma patients, who have significant immune perturbation. Whole-blood samples were aliquoted, and plasma was isolated, at days 0, 1, 2, and 3 after collection. Multiplexing techniques were used to measure protein concentrations, and general estimating equations were used to determine if there was a significant change over time. Over the 3-day period examined, only 15 of the 41 proteins showed no significant change in either cohort. Among the remaining proteins both increases and decreases were observed, with changes ranging from 2.4% per day to 325% per day. Proteins with significant changes in one cohort did not always show significant changes in the other group. These results support the need to separate plasma or serum from whole blood as quickly as possible and/or to standardize the length of time to processing within a given study of peripheral blood protein concentrations. When this is not possible, care should be taken to account for differences due to sample age.
Explanation:
Answer:
Under the basket.
Explanation:
Because he is under the basket, it is harder to miss.
Any object thrown upwards or dropped from a height<span> is pulled towards the Earth's surface due to Earth's gravity. Such objects experience a constant acceleration, known as acceleration due to gravity (</span>denoted<span> by g), which has a numerical value of 9.81 m/s^2.</span>
“Not only the above but also a heavy bag doesn't move, depending on your style you may end up chasing or running away a lot during a match.
The heavy bag is just punching with a little movement here and there in the ring your work rate has to be much higher otherwise your doomed.
Plus when you take punches that can fatigue you.”
honestly found this on yahoo answers lol, hope this helps!!
