A Scientific Hypothesis Must Be "Falsifiable". A scientific hypothesis must be testable, but there is a much stronger requirement that a testable hypothesis must meet before it can really be considered scientific
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Explanation: Denitrifying bacteria are the agents of this process. These bacteria use nitrate instead of oxygen when obtaining energy, releasing nitrogen gas to the atmosphere. Please let me know if this helps you!
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After this treatment, the investigators should expect to get a mixture of the desired enzyme, plus fragments of the peptide used to desorb the enzyme in question.
This would be the result of using a peptide as a desorption solution when the desired protein is a protease,
Assuming that the protease retains its activity in the medium in question, and that the peptide can act as a substrate (which would make sense), as the peptide solution is added, it will interact with and bind to the antibody, but some molecules will also interact with the active site of the enzyme as it desorbs and passes through, culminating on the elution of the hydrolized part of the peptide along with the enzyme.