<span>(C)Polar nucleus hope this helps
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Answer:
c. Prevent crystal violet from leaving the cells
Explanation:
Gram Staining:
- Gram staining is a staining techniques used for the identification of bacteria in microbiological studies.
- Gram staining uses crystal violet as the primary stain. It complexes with the peptidoglycan cell wall of Gram positive bacteria giving them a purple color.
- An iodine mordant is also used that forms a crystal violet-iodine complex that fixes the crystal violet dye to the cell wall. This prevents the Gram positive cell walls from being decolorized by the application of alcohol.
- A counter stain, safranin is then applied that stains Gram negative bacteria pink.
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