The enzyme Topoisomerase functions to prevent super-coiling of the DNA molecule during replication.
Explanation:
During replication, topoisomerases are required to relieve the positive supercoiling that arises from DNA unwiding mediated by Helicases.
A DNA topoisomerase is a nuclease that breaks a phosphodiester bond in a DNA strand.
All topoiomereases are classified as Topoisomerase I and Topoisomerase II depending on whether they cleave one or two strands respectively.
Topoisomerase I do not use ATP as a source of energy, the reaction is powered by energy stored in super-coiled DNA.
Topoisomerase II also do not require external energy but it utilizes ATP hydrolysis to drive conformation changes in the protein during reaction cycle.
Solar and geothermal energy are both considered renewable energy sources. ... Nuclear energy produces nuclear waste, while solar and geothermal energy do not produce a significant amount of waste.
