Answer:
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The answer to this question is <span>membrane potential
The gradient of electrolyte concentration from inside cell and outside cell will determine the membrane potential. Natrium or sodium is much higher outside the cell than inside cell. In contrast to the sodium, potassium is much more inside cells. This two electrolyte have major role in the membrane potential and action potential. </span>
The method used here is agarose gel elecrtrophoresis in which DNA ladder is used which is a set of standard used to determine the size of DNA used .The DNA ladder is put adjacent to the DNA fragments to be visualized Suppose if the ladder shows 300 basepair so the DNA adjacent to this number will be of this size.These are the set standards used to determine the size of DNA or RNA. For a range we can take 2% of agrarose to run DNA fragments upto 500 Basepair. Most of the amplified DNA falls into this category size.
<h2>Bacterial chromosome
</h2>
The DNA of most bacteria is contained in a single circular molecule, called the bacterial chromosome. The chromosome, along with several proteins and RNA molecules, forms an irregularly shaped structure called the nucleoid. This sits in the cytoplasm of the bacterial cell
