Eukaryotes are known to be bigger due to more complex organelles taking up space.
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Three lines of evidence that support the endosymbiotic theory:
1. The presence of membrane- Organelles like mitochondria and plastids have their own cell membranes, just like a prokaryotic cell does,
2. The presence of DNA- Each of these organelles have its own DNA genome, like a bacteria's genome, but much smaller.
<span>3. Reproduction (division)-Multiplication of the organelles occurs just like in bacteria, by division.</span>
It contains a cellular membrane which separates the interior of the cell from its environment.
Because Spain held such a dominant presence in the America's, Portugal focused on Africa. The correct option among all the options given in the question is option "A". The Spanish empire back then was one of the largest empire among all the empires. They were the masters of exploring the world via sea route and so they had created colonies far before the other. In America the Spanish were already present and expanding when Portugal decided to look elsewhere for creating colonies.