Answer:
You are interested in obtaining Staphylococcus aureus for a study investigating the prevalence of methicillin-resistant Staphylococcus aureus in the general population. You have received several samples and are ready to start your isolation procedures. Describe the personal protective equipment that would be needed and three different culturing techniques that can be used to obtain organisms to produce pure cultures. State if you use general or selective media and which specific media you would choose. How would you determine if the culture was contaminated? What is the first step you would take if you detected contamination?
Personal Protective equipment needed; No special precautions are necessary when working with methicillin-resistant staphylococcus aureus, staphylococcus aureus is a bio-safety level
Three different culturing media; to include;
- Etest Method
- Disc Diffusion
- Latex Agglutination.
Properly enriched selective media is used.
The most accurate method for identifying Merthicillin-resistant staphylococcus is to test for the presence of mecA gene by PCR.
The best specific media is latex agglutination test and oxacillin salt agar screen.
How to determine if the culture was contaminated;
It can be spotted by the turbidity of the growth medium or the floating branching mycelia, bacteria contamination can be detected under 10× microscope within few days of contamination.
Step to take if contamination is detected;
Biological contamination is a serious issue and being able to correctly identify different types of contamination is an essential first step in ensuring the safety of your culture.
Explanation:
The literature on methicillin susceptible testing is extensive, and often conflicting in recommendations regarding the most important method for routine use. this is partly because the various studies of phenotypic methods have included different strains, which may differ significantly in heterogeneity and behave differently under particular test condition.
<span>The peritoneal organs such as the intestines, liver, stomach, and gall bladder are covered by the peritoneum. This is a membrane that supports the organs by allowing nerves, blood vessels, and lymph vessels to pass through the organs. Without membranes, the proper functions of these organs will be impossible.</span>
One possible negative side effect of planting corn that is resistant to weed-killer is that: the resistance gene could be transferred to actual weeds, making farming more difficult.
<h3>What is genetic engineering?</h3>
Genetic engineering is also referred to as genetic modification and it can be defined as a technological process that involves the use of laboratory-based technologies to alter or modify the deoxyribonucleic acid (DNA) makeup of a living organism, in order to improve on both its physical and chemical conditions, or cure (correct) a genetic disorder in some cases.
Based on historical records, corn is a genetically modified crop with an ability to resist weed-killer, which makes it possible for it survive even when it is exposed to herbicide that are designed and developed to kill weeds.
However, one of the demerits of planting a genetically modified corn that is resistant to weed-killer is that the genes responsible for this resistance could be transferred to actual weeds, which would eventually make farming to be more difficult for farmers.
Read more on genetic engineering here: brainly.com/question/29330369
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<span>B.The results that support the claim are replicable by other scientists who follow the same procedures.
</span><span>Replicable is one strong characteristic of a study that can intensify the reliability and validity of the field, however using the same procedures as the experiementation done beforehand is critical to observe the same possible outcome.<span>
</span></span><span>They utilize and make use of the scientific method in order to have clear basis and evidence for their investigations. Research method is always used to answer every scientific inquiry and in gaining evidential data or knowledge. The scientific method has the following process or at least undergoes the process of
1. Observation</span>
2. Hypothesis
3. Experimentation
4. Interpretation of data
5. Evaluating the data
6. Passing and recording the data
<span>These steps are crucial and the empirical data that these scientists obtain are very important to keep that is why research paper, thesis and dissertations exists.<span> </span></span>
