1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Gnesinka [82]
2 years ago
5

You are interested in obtaining Staphylococcus aureus for a study investigating the prevalence of methicillin-resistant Staphylo

coccus aureus in the general population. You have received several samples and are ready to start your isolation procedures. Describe the personal protective equipment that would be needed and three different culturing techniques that can be used to obtain organisms to produce pure cultures. State if you use general or selective media and which specific media you would choose. How would you determine if the culture was contaminated? What is the first step you would take if you detected contamination?
Biology
1 answer:
Tanzania [10]2 years ago
4 0

Answer:

You are interested in obtaining Staphylococcus aureus for a study investigating the prevalence of methicillin-resistant Staphylococcus aureus in the general population. You have received several samples and are ready to start your isolation procedures. Describe the personal protective equipment that would be needed and three different culturing techniques that can be used to obtain organisms to produce pure cultures. State if you use general or selective media and which specific media you would choose. How would you determine if the culture was contaminated? What is the first step you would take if you detected contamination?

Personal Protective equipment needed; No special precautions are necessary when working with methicillin-resistant staphylococcus aureus, staphylococcus aureus is a bio-safety level

Three different culturing media; to include;

  • Etest Method
  • Disc Diffusion
  • Latex Agglutination.

Properly enriched selective media is used.

The most accurate method for identifying Merthicillin-resistant staphylococcus is to test for the presence of mecA gene by PCR.

The best specific media is latex agglutination test and oxacillin salt agar screen.

How to determine if the culture was contaminated;

It can be spotted by the turbidity of the growth medium or the floating branching mycelia, bacteria contamination can be detected under 10× microscope within few days of contamination.

Step to take if contamination is detected;

Biological contamination is a serious issue and being able to correctly identify different types of contamination is an essential first step in ensuring the safety of your culture.

Explanation:

The literature on methicillin susceptible testing is extensive, and often conflicting in recommendations regarding the most important method for routine use. this is partly because the various studies of phenotypic methods have included different strains, which may differ significantly in heterogeneity and behave differently under particular test condition.

You might be interested in
Anaerobic prokaryotes use ____to obtain energy.
Murrr4er [49]
<span>Pokaryotes are single cell organisms. Anaerobic prokaryotes use oxygen to obtain energy.</span><span> They use oxygen to perform cellular respiration, so they perform anaerobic respiration.
</span>
<span>Archaea and eubacteria are prokaryotic in nature. One of the differentiating characteristics between them is that eubacteria have peptidolycan in their cell walls.</span>
5 0
3 years ago
Read 2 more answers
Cationic detergents are surface active agents, also known as ______, which damage bacteria by binding bacterial surface proteins
vfiekz [6]
Surfactants; cell membrane
8 0
2 years ago
True or false: it is necessary to have a nervous system to sense and respond to the environment.
Mila [183]
Hello!

This would be false as it is just nerves inside of the body impulsing between each other and that would not be necessary to adapt to a new environment. 

I hope this helped!

I am, yours most sincerely,
SuperHelperThingy
5 0
3 years ago
Some students at a middle school conducted a laboratory investigation to learn more about the physical properties of different e
TiliK225 [7]

Answer:

A

Explanation:

1. The colour is non metallic.

2. properties of metal solidness is not there, because of results of hammer experiment.

3. Physical consistency is not solid.

Etc.

4 0
3 years ago
How does the heart detect changes in body activity?
madreJ [45]
By circulation of blood
8 0
3 years ago
Other questions:
  • The hawaiian islands are the top of an ocean ____
    12·1 answer
  • A close and permanent association between organisms of different species is called
    5·1 answer
  • Which cell organelles play a role in energy production by either building food molecules or breaking them down?
    6·1 answer
  • How is radioactive decay used to date sedimentary rocks?
    8·2 answers
  • What is the difference between multipolar, bipolar, and unipolar neurons?
    13·1 answer
  • Which of the following represents the correct flow of air into the lung of a mammal?
    13·1 answer
  • Should biofuels be used as a replacement for fossil fuels?
    7·2 answers
  • What do you think would happen if a genetic mutation caused a person's digestive tract to have stratified columnar epithelium in
    8·1 answer
  • When the cell excretes sodium from within against the natural tendency of matter it is performing
    11·1 answer
  • Nuclear-generated power plants are heavily subsidized. Do you think the market price of these plants should include the costs of
    14·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!