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Gnesinka [82]
3 years ago
5

You are interested in obtaining Staphylococcus aureus for a study investigating the prevalence of methicillin-resistant Staphylo

coccus aureus in the general population. You have received several samples and are ready to start your isolation procedures. Describe the personal protective equipment that would be needed and three different culturing techniques that can be used to obtain organisms to produce pure cultures. State if you use general or selective media and which specific media you would choose. How would you determine if the culture was contaminated? What is the first step you would take if you detected contamination?
Biology
1 answer:
Tanzania [10]3 years ago
4 0

Answer:

You are interested in obtaining Staphylococcus aureus for a study investigating the prevalence of methicillin-resistant Staphylococcus aureus in the general population. You have received several samples and are ready to start your isolation procedures. Describe the personal protective equipment that would be needed and three different culturing techniques that can be used to obtain organisms to produce pure cultures. State if you use general or selective media and which specific media you would choose. How would you determine if the culture was contaminated? What is the first step you would take if you detected contamination?

Personal Protective equipment needed; No special precautions are necessary when working with methicillin-resistant staphylococcus aureus, staphylococcus aureus is a bio-safety level

Three different culturing media; to include;

  • Etest Method
  • Disc Diffusion
  • Latex Agglutination.

Properly enriched selective media is used.

The most accurate method for identifying Merthicillin-resistant staphylococcus is to test for the presence of mecA gene by PCR.

The best specific media is latex agglutination test and oxacillin salt agar screen.

How to determine if the culture was contaminated;

It can be spotted by the turbidity of the growth medium or the floating branching mycelia, bacteria contamination can be detected under 10× microscope within few days of contamination.

Step to take if contamination is detected;

Biological contamination is a serious issue and being able to correctly identify different types of contamination is an essential first step in ensuring the safety of your culture.

Explanation:

The literature on methicillin susceptible testing is extensive, and often conflicting in recommendations regarding the most important method for routine use. this is partly because the various studies of phenotypic methods have included different strains, which may differ significantly in heterogeneity and behave differently under particular test condition.

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The chemical requirements for microbial growth include carbon, nitrogen, phosphorus, and sulfur. Why do all cells need nitrogen?
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Answer: For building amino acids, proteins and nucleic acids.

Explanation:

Living cells requires nitrogen because they are major part of amino acids. They form the building block of protein and nucleic acids such as DNA which carry the genetic information and is passed from generations to generations. Plants and animal need nitrogen for growth and survival. The protein is use for building and repairing muscles and it is a necessity for growth.

4 0
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The light micrograph shows dividing cells near the tip of an onion root. Identify a cell in each of the following stages: propha
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Mitotic division in onion root cells:

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  2. Metaphase: chromosomes line up and line up in the middle of the equatorial plane. The centromere of the chromosome appears to have bonds with the kinetochores connected to the spindle threads.
  3. Anaphase: the centromere of the chromosome undergoes division then the chromosomes are pulled by the spindle threads towards the two opposite poles and leave the center of the equator that looks empty.
  4. Telophase: The cytoplasm divides into two identical-looking daughter cells. At the telophase stage, the process of cell division occurs in two parts and has identical properties.

Mitosis is a cell division through the stages of prophase, metaphase, anaphase, and telophase. The purpose of mitosis is for growth and regeneration which results in two daughter cells that are identical to the original parent cell. Mitosis occurs only once and lasts only during somatization.

Learn more about mitosis here brainly.com/question/1186551

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7 0
1 year ago
using a genetic code chart and the mRNA codons provided in the table below, fill in the appropriate amino acids in the BLANK box
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Answer: easy

Explanation:

6 0
3 years ago
Some protists can reproduce both sexually and asexually. What's a major advantage of each?
Neporo4naja [7]

Answer:

(A). Sexual reproduction increases genetic diversity, and asexual reproduction can be more rapid.

Explanation:

Sexual reproduction is a mode of reproduction, which involves formation of new organisms by combination of genetic information from two organisms of different sexes. The major advantage of sexual reproduction is to develop genetic diversity as new organism is produced by mixing up genetic material of both the parents.

On the other hand, asexual reproduction involves formation of new organisms from a single parent having identical genetic makeup as present in parent cell.  One of the major advantage of asexual reproduction is to produce high number of offspring in less time as it is more rapid than sexual reproduction.

Thus, the correct answer is option (A).

6 0
3 years ago
Read 2 more answers
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