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mina [271]
3 years ago
11

Complete the flowchart to show the steps required to analyze gene activity using a microarray. preparing the cdna probe

Biology
1 answer:
Scilla [17]3 years ago
7 0
1- Preparing the cDNA:
  a- Using two different tissue samples (such as control and cancer cells), isolate mRNA.
  b- For each sample, prepare complementry DNA (cDNA) from the isolated mRNA. Then fluorescent label cDNA of each sample with a different color and then mixed.

2- preparing the microarray:
  a- Bind DNA fragments of different genes to microarray wells.
  b- Attach ssDNA (single-stranded DNA) to microarray wells.

3- Combine the probe and the microarray samples:
Add labeled cDNA to the microarray wells. 

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