Complete the flowchart to show the steps required to analyze gene activity using a microarray. preparing the cdna probe

Biology

1 answer:

Scilla [17]3 years ago

7 0

1- Preparing the cDNA:
a- Using two different tissue samples (such as control and cancer cells), isolate mRNA.
b- For each sample, prepare complementry DNA (cDNA) from the isolated mRNA. Then fluorescent label cDNA of each sample with a different color and then mixed.

2- preparing the microarray:
a- Bind DNA fragments of different genes to microarray wells.
b- Attach ssDNA (single-stranded DNA) to microarray wells.

3- Combine the probe and the microarray samples:
Add labeled cDNA to the microarray wells.

You might be interested in

Marmots are a type of _______. a. canine b. feline c. squirrel d. nocturnal predator

gavmur [86]

C. because mots are squriles that are very large lol is that all you needed?

4 0

3 years ago

Read 2 more answers

Cleaner fish feed off the algae, fungi, and other microorganisms that are found on larger fish. other species of fish, which loo

yulyashka [42]

Out of the following given choices;

A.microorganisms on large fish would decrease

B.cleaners and mimics would interbreed

C.behavior of large fish would change

D. cleaner population would increase

The answer is C. This is because, as a natural mechanism to defend themselves,the larger fish would become averse to all cleaner fish and cleaner-fish-looking fishes because it is difficult to differentiate between the beneficial and attacker fishes. Ultimately the cleaner fish would decrease and the larger fish would become more infested with parasites on their bodies.

7 0

3 years ago

A client is taking a monoamine oxidase (mao) inhibitor. the nurse plans care, knowing which information?

Nuetrik [128]

<span>The nurse would be aware that hypertension, nausea, vomiting, headaches and tachycardia are all indicators of hypertensive crisis. This is when the client has eaten foods that contain tyramine and tryptophan while taking MAO inhibitors. These specific meds lower the MAO in the liver, something that is very useful when the liver is breaking down tyramine and tryptophan. If the patient goes into hypertensive crisis, this can lead to the collapse of the circulatory system, intracranial hemorrhage, and even death.</span>

4 0

3 years ago

Blank is a blank found in the cell

Svetach [21]

We need more info to answer this chief

6 0

3 years ago

<img src="https://tex.z-dn.net/?f=what%20%5C%3A%20is%20%5C%3A%20cell%20%5C%3A%20membrane%20%5C%3A%20%20%20%5C%3A%20%7B%3F%7D" id

-Dominant- [34]

Answer:

Plasma membrane is the outermost layer in cells. It separates the content of cell from their external environment. ... It controls the entry and exit of materials in nd out of cell and hence it is called selectively permeable membrane.

8 0

3 years ago