Complete the flowchart to show the steps required to analyze gene activity using a microarray. preparing the cdna probe
1 answer:
1- Preparing the cDNA:
a- Using two different tissue samples (such as control and cancer cells), isolate mRNA.
b- For each sample, prepare complementry DNA (cDNA) from the isolated mRNA. Then fluorescent label cDNA of each sample with a different color and then mixed.
2- preparing the microarray:
a- Bind DNA fragments of different genes to microarray wells.
b- Attach ssDNA (single-stranded DNA) to microarray wells.
3- Combine the probe and the microarray samples:
Add labeled cDNA to the microarray wells.
a- Using two different tissue samples (such as control and cancer cells), isolate mRNA.
b- For each sample, prepare complementry DNA (cDNA) from the isolated mRNA. Then fluorescent label cDNA of each sample with a different color and then mixed.
2- preparing the microarray:
a- Bind DNA fragments of different genes to microarray wells.
b- Attach ssDNA (single-stranded DNA) to microarray wells.
3- Combine the probe and the microarray samples:
Add labeled cDNA to the microarray wells.
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