s would be secondary Explanation:
just cause
Answer:
Option 3 and 4 are most likely correct
Explanation:
<em>Option 3</em>: We know that area 1 would contain heavy streaking and not single colonies. Therefore, if the loop crosses area 3 and enters area 1, it will definitely result in heavy streaking again.
<em>Option 4</em>: We always need to sterilize properly the loop when streaking in different areas. If we don't do it, it is possible that the loop contains a lot of bacteria that would be streaked again.
Options 1 and 2 are incorrect because, according to question, there is a uniform growth but no isolated colonies. So, contamination or mixed culture would not produce uniform growth streaking rather mixed with other bacterial types.
A)amino acid is the correct answer
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