3 years ago

How to calculate concentration with just the absorbance and % transmittance measurements from a spectrometer?

Mathematics

1 answer:

Natali [406]3 years ago

8 0

Answer:

A=ε*l*c

A= 2- log₁₀ % T

Step-by-step explanation:

There is a linear relationship between the concentration of a sample and absorbance according to Beer-Lambert Law.

A=ε*l*c

where;

A=absorbance

ε=absorption coefficient

l=path length

c=concentration

Because % transmittance is transmittance value multiplied by 100 then, the equation that will allow us calculate absorbance from % transmittance value will be;

A= 2- log₁₀ % T where T is transmittance.

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