Question

Suppose you are a scientist trying to help people who cannot produce an enzyme needed for proper digestion.How could you use genetic engineering techniques to make transformed bacteria that produce the enzyme?

Answer 1

By using genetic engineering techniques to transform bacteria that produce the enzyme I will extract the DNA from the cells of different people who can make the enzyme digestion. Then will cut the DNA with a restriction enzyme, and use gel electrophoresis and a DNA probe to locate the gene. By using the polymerase chain reaction I will copy the gene. Thereafter, I will choose a plasmid that has an antibiotic resistance genetic marker, and cut the plasmid with the same restriction enzyme used to cut out the human gene.
Later I will insert the copies of the human gene into the plasmids and allow the bacterial cells to take in the plasmids. Later, I will allow the bacteria to grow in a culture containing the antibiotic. And hence, these bacteria will make the digestion enzyme.