To clone the entire coding region of p53 into the plasmid, which restriction enzyme(s) could you use to cut both your PCR produc

Question

3 years ago

7

To clone the entire coding region of p53 into the plasmid, which restriction enzyme(s) could you use to cut both your PCR produc

t and the pCDNA3 plasmid? Select all that apply. Select all that apply. E (EcoRI) B (BamHI) H (HindIII) S (SmaI) X (XbaI) N (NotI)

Biology

1 answer:

Sergio039 [100] · Answer 1 · 2022-07-31T01:23:46+03:00

Answer:

E (EcoRI) B (BamHI) H (HindIII) X (XbaI) N (NotI).

Explanation:

Only <em>Sma</em>I does not have a recognition site in the polylinker of pCDNA vector (See map attached). So, in theory, any of the other enzymes: E (EcoRI) B (BamHI) H (HindIII) X (XbaI) N (NotI) could be used. It would be of importance to see in silico the digestion of regions flanking the p53 gene.