Answer;
-It belongs to Domain archaea
Explanation;
-Domain Archaea is any of a group of single-celled prokaryotic organisms (organisms whose cells lack a defined nucleus) that have distinct molecular characteristics separating them from bacteria as well as from eukaryotes.
-Eukaryote and archaea (ancient bacteria) both have DNA wrapped around histones to form chromatin.
-Eubacterium (true bacteria) do not contain histones.
-Eukaryotes have multiple linear chromosomes while archaea have a single circular chromosome.
The hedgehog ligans activate it
BIOTIC FACTORS OF DESERT:
-Cacti
-other plants mesquite
-rattlers
-desert tortoise
-desert rats
-hawks
-peccary
-licasrds
Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
