Under high-power lens, onion root tip appear as large, rectangular interlocking cells. It is clearly visible that cells are surrounded by distinct cell walls. Each cell has a dark stained nucleus. Large vacuoles are also observed in the cells. Small starch granules are also present inside the cells within the cytoplasm. Since root cells do not take part in photosynthesis, no chloroplasts are observed.
Answer:
DNA restriction enzymes cut the DNA molecule, while DNA ligases join the resulting DNA fragments
Explanation:
Transformation is a naturally occurring process by which bacteria incorporate exogenous genetic material from their surrounding environment. This process (transformation) is used for DNA cloning via plasmid vectors. In DNA cloning, transformation occurs after restriction enzymes cut the DNA at specific sequences named palindromic sequences (i.e, sequences that can be read the same in opposite direction). Restriction enzymes can generate sticky-ends, where enzymes make staggered cuts in the two strands (e.g., <em>BamH</em>), or blunt ends, where the resulting strands are of the same length (e.g., <em>HaeIII</em>). In general, sticky-end enzymes are more useful because they generate a 3' overhang in one molecule and a complementary 5' overhang in the other, increasing the yield and specificity of ligation. During ligation, a DNA ligase is used to join both DNA strands by forming phosphodiester bonds in the plasmid. Following transformation, bacteria can be selected on antibiotic plates.
It would be the male because because it developes more than in a female. in the female it degenerates.
