Charlidamelio is overrated
Answer:
need to be done with plenty of observation to avoid infection.
Explanation:
This technique is quite delicate because the main risk is infection. Some of the main risks are neuromuscular disease, sedation or neurological illness.
Another risk is that by passing the time, there is a difficult in respiratory, in this case, the main risk is directly to the heart, with some stoke, due to the high concentration of carbon dioxide due to the low exchange among oxygen and CO2.
Some of the indications are:
a.- Coarse crackles auscultated over trachea.
b.- Increase the respiratory pressure.
c.- Decrease tidal volume.
d.- Check the levels of oxygen in blood as in arteries.
e.- Check that patients can generate a cough.
Hope this info is useful.
It would be B. i hope i helped you out, have a nice day
The answer is D. For producing recombinant, first you need to cut the insert part and vector part to get the same end by using restriction enzyme. Then using DNA ligase to bind these two parts to get a new plasmids.