The answer is B. Cross-pollination occurs when pollen fertilizes female egg cells on the same flower.
Answer:
Enzymes serve as catalysts to many biological processes, and so they are not used up in reactions and they may be recovered and reused. However, in a laboratory setting, reactions involving enzymes can leave the enzyme unrecoverable. This process makes the enzyme at once less reactive but more stable.
Answer:
Background
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
Explanation:
Results
Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
The correct answer is "limited prey is driving the bears to seek food in human settlements".
Due to the warming of the Arctic waters, the ice is melting, resulting in a serious decline of floating winter ice. Polar bears need these floating parts of ice to hunt, therefore this new situation limits the hunting opportunities of the bears. That is why polar bears are driven to human settlements to look for food.
