Question

Which reactions in glycolysis are physiologically irreversible?

Answer 1

The reactions of glycolysis are made up of ten steps, out of the ten step three are irreversible, this is because, these reactions have large negative free energies The three steps that are irreversible are:
1. Phosphorylation of glucose.
2. Phosphorylation of glucose 6 phosphate and 
3. Transfer of phosphate from phosphoenolpyruvate to ADP.