Type II restriction enzymes, such as Ecor I, that make staggered cuts within its recognition sequence, are considered more effective in biotechnology because they result in cohesive or sticky ends.
<h3>What is Ecor I and why are sticky ends important?</h3>
Ecor I is a kind of restriction enzyme which is obtained from Escherichia coli. The palindromic sequence recognized by this enzyme is 5' - GAATTC - 3'. It makes the following cuts between G and A on both the strands of the DNA to form sticky ends:
5' - G↓AATTC - 3'
3' - CTTAA↑G - 5'
Sticky ends are a fragment of DNA which is produced through a staggered cut, by the use of restriction enzyme. In this the terminal portion stretches with unpaired nucleotides. These kind of ends are easy to ligate when rDNA needs to be formed.
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