Your research group is investigating the possible use of genetically - engineered cells to produce a vaccine for malaria. In par agraph form, explain the pros AND cons of using E. coli, Saccharomyces, AND animal cells to produce the vaccine.
1 answer:
Answer:
<u>Attributes of E. coli articulation frameworks </u>
Advantages:
Quick articulation
Simplicity of culture
Significant returns
Cheap
Genome alterations conceivable
Large scale manufacturing quick and practical
Disadvantages:
Proteins with disulfide bonds hard to communicate Produce unglycosylated proteins Proteins created with endotoxins Acetic acid derivation development bringing about cell lethality
Proteins created as consideration bodies produce dormant proteins needs collapsing
<u>YEAST SYSTEM </u>
Advantages:
Nearness of post translational change discharge can be recognized by emission signal
develop in minimal effort media straightforward hereditary control
Disadvantages:
<u>Bacillus articulation frameworks </u>
Advantages:
Solid discharge
no association of intracellular consideration bodies Simplicity of control Hereditarily all around portrayed frameworks Exceptionally created change and quality substitution advancements. Unrivaled development qualities financially savvy recuperation
<u>Animal Cells: </u>
Advantage:
nearness of post interpretation adjustment
Disadvatages
Issues with creature utilization
Can get sullied with creature diseases
Exorbitant downstream preparing
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