Question

Your research group is investigating the possible use of genetically - engineered cells to produce a vaccine for malaria. In paragraph form, explain the pros AND cons of using E. coli, Saccharomyces, AND animal cells to produce the vaccine.

Answer 1

Answer:

Attributes of E. coli articulation frameworks  

Advantages:

• Quick articulation  
• Simplicity of culture  
• Significant returns  
• Cheap  
• Genome alterations conceivable  

• Large scale manufacturing quick and practical  

Disadvantages:

• Proteins with disulfide bonds hard to communicate  
• Produce unglycosylated proteins  
• Proteins created with endotoxins  
• Acetic acid derivation development bringing about cell lethality  
• Proteins created as consideration bodies  
• produce dormant proteins  
• needs collapsing  

YEAST SYSTEM

Advantages:

• Nearness of post translational change  
• discharge can be recognized by emission signal  
• develop in minimal effort media  
• straightforward hereditary control  

Disadvantages:

• Low yeild of item  

Bacillus articulation frameworks  

Advantages:

• Solid discharge  
• no association of intracellular consideration bodies  
• Simplicity of control  
• Hereditarily all around portrayed frameworks  
• Exceptionally created change and quality substitution advancements.  
• Unrivaled development qualities  
• financially savvy recuperation  

Animal Cells:

Advantage:

• nearness of post interpretation adjustment  

Disadvatages

Issues with creature utilization  

Can get sullied with creature diseases  

Exorbitant downstream preparing