Non-fluorescent calcein AM is hydrolyzed by intracellular esterases into the green fluorescent dye calcein, which can be used to quantify the number of viable cells.
https://www.creative-bioarray.com/support/calcein-am-cell-viability-assay.htm
Answer:
1. Wind
When strong winds blow, the topsoil along with the organic matter is carried away by the wind. This happens more often when the land is not covered with grass or plants. Such conditions are very common in desert and semi-desert regions where strong winds blow very frequently.
2. Water
When it rains in the hilly areas, the soil gets washed away towards the plains. The running water deposits the mineral-rich soil in the riverbed and over the years this deposition of soil can change the course of the river. This can lead to floods which cause the destruction of life and property. Water erosion leads to loss of agriculture potential.
3. Overgrazing
When cattle are allowed to graze on the same field repeatedly, all the available grass, including the roots are eaten by them. This makes the topsoil vulnerable to wind and flowing water, leading to soil erosion.
4. Deforestation
Humans have taken land from the forest to cultivate in order to feed the ever-increasing population and to build houses, industries, etc. Cutting down of trees on a large scale for these purposes is deforestation. The roots of trees hold the soil together, thus preventing the soil from getting uprooted. When large areas of the forest are cleared, the topsoil gets eroded by wind and flowing water.
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Energy comes from making bonds in carbon dioxide and water.
A change in protein comes along.
I hope this helps and have a wonderful day filled with joy!!
