In Eukaryotes, the DNA replication can start at several sites or places in the DNA molecule or DNA strand. The only important factor for DNA replication to start is that it starts at replication fork.
The replication of DNA is semi-conservative in nature because each strand acts as a template for synthesizing a new complementary strand of the double helix.
The new DNA is made with the help of the enzyme DNA polymerase which requires a primer for starting the DNA synthesis.
Replication occurs only at specific regions of DNA known as origin of replication and these are short sequences of nucleotides which are recognized by the enzyme for the replication.
The special enzymes or proteins recognize the specific sites and then binds to them. After binding, the DNA is opened up forming the replication forks which are two Y- shaped structures.
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