Answer:
the first stop is used to fill the micropipette tip and the second stop is used to dispense the content of the tip
The assumption in this problem is that the gas, CO2, is ideal and that the ideal gas law is applicable such that,
n = PV/RT
We simply substitute the given values to the equation above,
n = (1 atm)(V) / (0.082 Latm/mol.K)(T)
n = 12.20 V/T
I believe the answer should be mates...(2)