what is the question and answer and i will help u
A certain drug is made from only two ingredients: compound A and compound B. There are 5 milliliters of compound A used for every 4 milliliters of compound B. If a chemist uses 255 milliliters of compound A, how many milliliters of the drug will be made?
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4:5 is the same as 4x:5x where 4x is compound A and 5x is compound B.
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Solve for "x":
If amount of compound A is 4x = 255
x = 255/4
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Then amount of compound B is 5x = 5(255/4) = 318.75 ml
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Cheers !!!
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Answer:
Because the leading strand is synthesized continuously while the lagging strand is discontinuously synthesized.
Explanation:
Two DNA strands have opposite polarity, that is, the 5' end of one strand lies opposite to the 3' end of the other strand. However, DNA replication can occur in 5' to 3' direction only since the formation of phosphodiester bonds require the presence of nucleophile 3' OH to attack the 5' phosphate of an incoming deoxyribonucleotide.
The problem is solved by the synthesis of the leading strand continuously and the synthesis of the lagging strand in a discontinuous manner. Lagging strand synthesis requires the formation of primers and their elongation to form Okazaki fragments that may have few hundreds to few thousands of nucleotides. Removal of primers from Okazaki fragments is followed by joining them together to make the long lagging strand. The discontinuous replication of the lagging strand makes the replication at the leading strand ahead of it.
You should mix
4µl of DNA sample and complete it with
96µl (100-4) of water.
You can figure this out by using
C1.V1 = C2.V2C1 = 250 <span>ng/μl (Sample Concentration)
</span>V1 = ?
(the necessary volume to take from the DNA sample)C2 = 10 <span>ng/μl (PCR concentration)
</span>V2 = 100 µl (total volume)
V1 =
![\frac{C2 . V2}{C1}](https://tex.z-dn.net/?f=%20%5Cfrac%7BC2%20.%20V2%7D%7BC1%7D%20)
=
![\frac{10 . 100}{250}](https://tex.z-dn.net/?f=%20%5Cfrac%7B10%20.%20100%7D%7B250%7D%20)
=
4µl
For the water: 100µl (total volume) = 4µl + Water
Water volume = 96µl