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Paladinen [302]
3 years ago
5

Which of the following reactions with covalently closed, circular DNA (cccDNA) does NOT result in a new linking number? A. incub

ation with a type II topoisomerase B. incubation with a type I topoisomerase C. incubation with ethidium D. brief incubation with DNase I followed by resealing the breaks in the
phosphodiester backbone with another enzyme (DNA ligase)
Medicine
2 answers:
alisha [4.7K]3 years ago
8 0

Answer:

Okay

Explanation:

Human topoisomerase I plays an important role in removing positive DNA supercoils that accumulate ahead of replication forks. It also is the target for camptothecin-based anticancer drugs that act by increasing levels of topoisomerase I-mediated DNA scission. Evidence suggests that cleavage events most likely to generate permanent genomic damage are those that occur ahead of DNA tracking systems. Therefore, it is important to characterize the ability of topoisomerase I to cleave positively supercoiled DNA. Results confirm that the human enzyme maintains higher levels of cleavage with positively as opposed to negatively supercoiled substrates in the absence or presence of anticancer drugs. Enhanced drug efficacy on positively supercoiled DNA is due primarily to an increase in baseline levels of cleavage. Sites of topoisomerase I-mediated DNA cleavage do not appear to be affected by supercoil geometry. However, rates of ligation are slower with positively supercoiled substrates. Finally, intercalators enhance topoisomerase I-mediated cleavage of negatively supercoiled substrates but not positively supercoiled or linear DNA. We suggest that these compounds act by altering the perceived topological state of the double helix, making underwound DNA appear to be overwound to the enzyme, and propose that these compounds be referred to as ‘topological poisons of topoisomerase I’

Natasha2012 [34]3 years ago
3 0

Answer:incubation with ethidium

Explanation:

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Part 1 Questions 1. What are the main symptoms of influenza? 2. Would you expect a flu shot to protect against the ""stomach flu
Anna11 [10]

Answer:

Influenza and stomach flu are caused by different viruses and cannot be prevented by similar vaccines. Moreover, infleunza infection or flu vaccine does not have any direct effect on autism. URIs infections may occur by different infectious agents and shall be treated accordingly. the Question wise answers are explained below.

Explanation:

1. Influenza can cause mild to severe illness and is different from cold. People suffering from flu has fever, chills, cough, sore throat, runny nose, headaches, muscle aches, and severe fatigue.

2. Flu shots are strongly recommended to protect against influenza but does not help with stomach flu also known as gastroenteritis. Stomach flu is an infection caused by rotaviruses and noroviruses, and is not caused by the Influenza virus. Hence, flu shot protecting the body from influenza virus is not effective in stomach flu or gastroenteritis.

3. Influenza virus attacks the respiratory system including nose, throat and lungs, whereas, gastroenteritis attacks the intestines. Hence both influenza and stomach flu may have similar as well as contrasting symptoms. Influenza causes coughing, congestion, fever, chills, headache and muscle aches. Gastroenteritis causes fever, chills, headache, body aches, vomiting and diarrhea. The risk of influenza and gastroenteritis can be reduced with washing hands often with disinfectant. Hygienic drinking water is also necessary to avoid stomach flu as it causes diarrhea and food poisoning. Flu shots are given annually for influenza virus protection and two oral vaccines of rotavirus are given to young infants for avoiding stomach flu or gastroenteritis.

4. Flu shots provides immunity against specific strains of flu virus including influenza A strain- H1N1 and H3N2, and influenza B strain.  The trivalent flu vaccines protect against both Influenza A strain, whereas, the Quadrivalent vaccine help against both the influenza A strains and also the influenza B strain. These flu shots are given through a needle, cell-based version, and in the form of a recombinant vaccine which does not contain the virus or eggs. The mild side effects produced after taking the influenza vaccine include low grade fever, aches, redness and swelling at the site of injection, soreness etc. which happens to occur in only 1-2% of people taking the vaccine.  

Serious allergic reactions may also occur in some people such as difficulty breathing, swelling of eyes or lips, high pulse, high fever and dizziness. Children may have runny nose, wheezing, fever, muscle ache, vomiting and headache as side effects to the flu vaccine.  

CDC suggests that influenza infection or influenza vaccines taken during pregnancy are not associated with risk of autism in fetus. The idea evolves from the fact that inflammatory cytokines may be produced from the injection of influenza virus in vaccine form. However, this proposition is not evident from clinical trials conducted on autistic children who are diagnosed and those who are at risk of autism.

5. The upper respiratory tract infection occurs in the sinuses, nasal passages, pharynx and larynx region. Hence, the upper airways is blocked and symptoms of inflammation in the tract, cold, congestion and difficulty in breathing occurs. URIs are either caused by viruses or by bacteria in some cases. The URIs are infectious and may spread from person to person through air and touching of objects exposed to virus. Coming in close contact with patient, poor hygiene and avoiding frequent hand wash, and low immunity in HIV etc. are some of the risk factors of URI.  

Antibiotics are not given in URIs unless a bacterial infection is the cause of airways blockage. Hence, URIs are generally treated with non-steroidal anti-inflammatory drugs (NSAIDs), acetaminophen, Antihistamines and cough medications. Steroids and decongestants are also given in patients who are suffering from severe airways blockage and congestion. In immune-compromised patients who are already suffering from other disease conditions, anti-bacterials and anti-viral antibiotics are given to treat the URIs.  

8 0
3 years ago
Someone be my friend PLEASEEE
mixas84 [53]

Answer:

.............

Explanation:

Okay I will be your friend

7 0
2 years ago
A deceitful act with the intention to conceal the truth
Vaselesa [24]

Answer:

Fraud

Explanation:

7 0
3 years ago
Here is how Lydia found the product 4/5 x 10. Use the model given to show that Lydia's answer is correct.
Veseljchak [2.6K]

Answer: Lydia's answer is correct.

Explanation:

The solution used by Lydia is:

4/5 × 10 = 4 × 1/5 × 10

= 4 × 10/5

= 4 × 2

= 8

The above solution by Lydia is correct.

Given the question, 4/5 × 10, this will be:

= 4/5 × 10/1

Let's multiply the numerator first, this will be: = 4 × 10 = 40

The denominator will be: = 5 × 1 = 5

Therefore, 4/5 × 10/1 = 40/5

Now when we divide 40 by 5, we will get 8. Therefore Lydia is correct.

4 0
3 years ago
Any help on 16&17 pl
egoroff_w [7]
16; D. all of the above

explanation; the gluteus maximus works with the semitendinosus and semimembranosus muscles, extending the thigh. it is, indeed, located in the buttocks, and can be used as an injection site.

17; D. both B and C

explanation; the latissimus dorsi collaborates with the teres major and pectoralis major to adduct and medially rotate the arm at it’s glenohumeral joint.
3 0
3 years ago
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