1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Paladinen [302]
4 years ago
5

Which of the following reactions with covalently closed, circular DNA (cccDNA) does NOT result in a new linking number? A. incub

ation with a type II topoisomerase B. incubation with a type I topoisomerase C. incubation with ethidium D. brief incubation with DNase I followed by resealing the breaks in the
phosphodiester backbone with another enzyme (DNA ligase)
Medicine
2 answers:
alisha [4.7K]4 years ago
8 0

Answer:

Okay

Explanation:

Human topoisomerase I plays an important role in removing positive DNA supercoils that accumulate ahead of replication forks. It also is the target for camptothecin-based anticancer drugs that act by increasing levels of topoisomerase I-mediated DNA scission. Evidence suggests that cleavage events most likely to generate permanent genomic damage are those that occur ahead of DNA tracking systems. Therefore, it is important to characterize the ability of topoisomerase I to cleave positively supercoiled DNA. Results confirm that the human enzyme maintains higher levels of cleavage with positively as opposed to negatively supercoiled substrates in the absence or presence of anticancer drugs. Enhanced drug efficacy on positively supercoiled DNA is due primarily to an increase in baseline levels of cleavage. Sites of topoisomerase I-mediated DNA cleavage do not appear to be affected by supercoil geometry. However, rates of ligation are slower with positively supercoiled substrates. Finally, intercalators enhance topoisomerase I-mediated cleavage of negatively supercoiled substrates but not positively supercoiled or linear DNA. We suggest that these compounds act by altering the perceived topological state of the double helix, making underwound DNA appear to be overwound to the enzyme, and propose that these compounds be referred to as ‘topological poisons of topoisomerase I’

Natasha2012 [34]4 years ago
3 0

Answer:incubation with ethidium

Explanation:

You might be interested in
An apparently intoxicated 23-year-old woman attempted to tackle her boyfriend by jumping from the handrail outside her second-st
True [87]

Answer: B) apply gentle traction to the bone injury and splint the ankle with a padded rigid splint.

Explanation:

Capillary refill time is the time taken by the capillary bed to change color from red to complete blanching. It can be measured by holding the hand to a level higher than the heart level and by applying pressure over the soft pad of fingers and observing the color change. Normal capillary refill time is 2 seconds.  

A capillary time of 4 seconds indicates that capillaries are not functioning properly and blood is not reached immediately over the feet. Thus application of traction can help by pulling the fractured body part.  The split is a medical device which can provide support to the ankle.

3 0
3 years ago
An intracerebral hemorrhage occurs when a blood vessel bursts, allowing blood to:
Oksanka [162]

Answer:

leak inside the brain

Explanation:

An intracerebral hemmorhage occurs when a blood vessel within the brain bursts, causing bleeding inside the brain.

Hope that helps.

3 0
3 years ago
The nurse is caring for a client receiving tamoxifen (nolvadex) for the treatment of breast cancer. which action should the nurs
Darya [45]

The action the nurse should include in the client's plan of care who is receiving tamoxifen (nolvadex) for the treatment of breast cancer is to help the client cope with hot flashes

Tamoxifen, a selective estrogen receptor modulator, is a form of hormonal therapy (SERM). The medication binds to breast cancer cells' hormone receptors (specific proteins). Once the drug is within the cells, it prevents cancer from getting access to the hormones it needs to proliferate and develop.

Tamoxifen prevents estrogen from binding to the receptor, which prevents the cancer cell from receiving signals from estrogen that would otherwise cause it to grow and reproduce. Hot flashes, exhaustion, an elevated risk of blood clots, and endometrial cancer are some of the side effects that this drug may cause.

To learn more about tamoxifen and hot flashes here,

brainly.com/question/13142326

#SPJ4

3 0
1 year ago
How do schema, assimilation, and accommodation work together to help kids understand their world?
gulaghasi [49]

Schema, assimilation and accommodation

Explanation

Schema, assimilation and accommodation are three important concepts in Piaget’s cognitive development which the kids adapt to learn and understand their world.

Schema is the mental or cognitive idea, concept or framework which organizes and interprets information. Schema is built continuously during cognitive development of children through the interweaving of the adaptation processes of assimilation and accommodation.  

Assimilation helps a kid to take new information and add it to the existing schema or old information about the same concept. For ex., identifying a four-legged animal as a cow by a kid is assimilation of a new information.

Accommodation helps a kid to analyze and modify the new or old schema or information based on what is learnt or experienced. For ex., Identifying that all four-legged animals are not cow and they can be a horse or bullock also depending upon the difference in their structure is the accommodation made by the kid on top of existing schema of four-legged animals.

5 0
3 years ago
Read 2 more answers
Cindy is an 11-year-old, 79 lb child who sliced her forearm open on a sharp piece of metal while helping her father clean out th
mylen [45]

Answer:

not safe

Explanation:

Because the package says the oral dose is only 0.25 to 1

5 0
3 years ago
Other questions:
  • One of the five components of fitness is muscular strength. This is the ability of muscles to __________________________________
    8·1 answer
  • This circular skeletal muscle surrounds the urethra as it passes through the pelvic floor and can be controlled consciously to e
    9·1 answer
  • Seeking prenatal care and avoiding unapproved medicine and drugs can lead to a miscarriage. Please select the best answer from t
    7·2 answers
  • How can you correct any steps you missed or did incorrectly?
    11·1 answer
  • What does democratized knowledge mean?
    12·1 answer
  • Prescriptions may state that a patient should be given "ibuprofen as indicated for pain." Which prescription gives the correct i
    14·2 answers
  • Many of the challenges faced by older adults may be minimized because they are a part of the expected life course, yet many olde
    9·1 answer
  • Which of the following statements is true? Multiple Choice An undercooked hamburger patty is the most likely source of giardia.
    10·1 answer
  • The nurse plans an activity for a chronically ill hospitalized child. which activity is the best choice to provide stimulation f
    14·1 answer
  • You must obtain consent before performing the heimlich maneuver on a conscious adult. True or false?.
    14·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!