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Ganezh [65]
3 years ago
12

Tentative explanation of a phenomenon that can be tested

Biology
1 answer:
stellarik [79]3 years ago
5 0
A tentative explanation of a phenomenon that can be tested I believe is a scientific experiment.
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If acetyl-CoA is labeled with 14C at its methyl group, how many rounds of the cycle are required before 14CO2 is released?
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Answer:

14 CO₂ will be released in the second turn of the cycle

Explanation:

<u>Complete question goes like this</u>, "<em>The CO2 produced in one round of the citric acid cycle does not originate in the acetyl carbons that entered that round. If acetyl-CoA is labeled with 14C at the carbonyl carbon, how many rounds of the cycle are required before 14CO2 is released?</em>"

<u>The answer to this is</u>;

  • The labeled Acetyl of Acetyl-CoA becomes the terminal carbon (C4) of succinyl-CoA (which becomes succinate that is a symmetrical four carbon diprotic dicarboxylic acid from alpha-ketoglutarate).
  • Succinate converts into fumarate. Fumarate converts into malate, and malate converts into oxaloacetate. Because succinate is symmetrical, the oxaloacetate can have the label at C1 or C4.
  • When these condense with acetyl-CoA to begin the second round of the cycle, both of these carbons are discharged as CO2 during the isocitrate dehydrogenase and alpha-ketoglutarate dehydrogenase reactions (formation of alpha-ketoglutarate and succinyl-CoA respectively).

Hence, 14 CO₂ will be released in the second turn of the cycle.

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