Answer and Explanation:
The steps for construction of the expression plasma
- STEP 1 : Introducing Notl and Hindlll confinement sites at the ends of the gene of interest by means of PCR
- STEP 2 :Digest the plasmid and DNA encoding gene with the Notl and Hindol confinement endonucleuses.
- STEP 3 : Ligate the cut plasmid and DNA fragment
- STEP 4 : Change the plasmid into E.coli for expression
- STEP 5 : Clean the protein by utilizing a column of immobilized

Answer: The options are not included.
But the sites are;
Interaction with ribosomes.
Interaction with aminoacyl tRNA
synthase.
Attachment of the specific Amino acid.
Interaction with codon.
Explanation:
Transfer RNA is a type of RNA that help to translate messenger RNA sequence into protein. Each tRNA have two major areas; the anticodon and region for attaching specific Amino acids.
tRNAs function at specific sites in the ribosomes during mRNA deciding.
The four specific recognition sites of trna that must be inherent in it's tertiary structures in order for it to carry out it's role are;.
Interaction with ribosomes.
Interaction with aminoacyl tRNA synthase.
Attachment of specific Amino acid.
Interaction with codon.
Answer:
According to Nutton, we are unable to identify any diseases familiar to us today because we are hampered by the great difference between ancient and modern understanding of the concept of 'a disease'.
The evidence or claim he makes to support this, is in his book "Seeds of Disease" where he states that during the ancient medicine practice, the interpretatation was not held nor rigorously or strict, employing words far looser metaphoric sense, interchangeably with what they had known from Galen instead.
Explanation:
Professor Vivian Nutton specialises in the history of the classical tradition in medicine, from Antiquity to the present, and particularly on Galen. He is currently co-editor of Medical History. Heirs of Hippocrates
, how they exercised their influence, and how they were received and interpreted over the centuries, are fascinating stories. It was taken over and translated into Latin, Arabic, Hebrew and a range of European languages.
His main work has focused around Galen of Pergamum (129–216/7 AD), the most prolific writer to survive from the ancient world, whose combination of great learning and practical skill imposed his ideas on learned doctors for centuries, and, secondly, on the development of medical ideas and practices in the Renaissance of the sixteenth century.
Answer:
D. Solid Ice Crystals
Explanation:
"Cirrus clouds are wispy, curly, or stringy. They are found high in the atmosphere—typically higher than 6,000 meters (20,000 feet)—and are usually made of ice crystals." -National Geographic
B) lake
Explanation:
A lake is natural body of still fresh or salt water usually found inland and sometimes in mountain regions. They are larger and deeper than ponds in all proportions.
- A lake can be viewed as body of water surrounded by land.
- Most lakes are usually internal drainage basins that collects water from rivers the feeds and empties into them.
- Example is Lake chad.
- Rivers do not go out of lakes, they are fed by rivers.
Learn more:
Lake practices brainly.com/question/3817191
#learnwithBrainly