Answer:
In this study, we tested the effects of NEAA-deprived diets and checkpoint inhibitor anti-PD-1 and anti-PD-L1 in colon cancer using syngeneic mouse model (Balb/c) bearing tumors of mouse colorectal cancer cell line CT-26. Three diets were tested, including a natural rodent diet Teklad ENVIGO Global 16% Protein Rodent Diet (control 1), a formulated NEAA-complete diet COMPLETE (control 2, using amino acid mix in place of protein), and a formulated NEAA-deprived diet FTN203 (treatment, using amino acid mix in place of protein). Both COMPLETE and FTN203 have the same nutritional structures, contain 17% w/w protein equivalent, and are isocaloric. After tumor size-based randomization, these diets were provided to mice ad libitum throughout the whole test. Each of these diets was used alone or combined with anti-PD-1 antibody (i.p., twice per week for 2 weeks) or anti-PD-L1 antibody (i.v., twice per week for 2 weeks).
Explanation:
Friedman and Johnson (1997) show that for a wide range of dynamic optimization problems, supermodularity is both necessary and sufficient for monotone static results. In the present context, this implies that our supermodular model requires the minimum set of assumptions to obtain monotonicity in the optimal decision variables.
2
The evidence presented here needs to be supplemented with information about inter- and intrafamily income transfers. This issue was addressed in a follow-up survey, but analysis of the results is not yet complete.
