Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
I think can be water, light, temperature, nutrients
Hope help!
Biological molecules or biomolecules are the carbohydrates, proteins, lipids and nucleic acids that are produced by the living organism. A very significant relationship lies between their structure and function as they are inter relatable.
Biomolecules are designed in such a way that on forming a particular structure only their specific functions can be formed. It is to ensure that no molecules other than the desired ones bring a change. If the latter situation arises, it may refer to harm or lethality.
For instance, insulin binds to its receptor and decreases the blood glucose level. Change in its structure will lead in inability to bind to the receptor specifically designed to accomodate it. And if function is not dependent on structure, then any molecule may bind to the receptor and decrease the blood sugar level to the point that body may not be able to function.
Learn more about biomolecules -
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Answer:
The second statement is correct: "The "host range" for a virus is determined by the presence or absence of particular components on the surface of a host cell that are required for the virus to attach."
Explanation:
The virus attaching itself to the host cell is the first step for it to successfully infect the host. Viruses are smaller in size than bacteria, so the first statement is incorrect. The third statement is incorrect because viruses do not have the necessary machinery to carry out protein synthesis or to generate ATP, this precisely why they seek out a host to begin with. The fourth statement is false because the virus will either have DNA or RNA, but both at the same time.
Hope that answers the question, have a great day!
1. The plant should not have leaves. To prevent transpiration from taking place
2. Plant should be well watered. Plants need water
3. Put some Vaseline to seal open gaps. This prevents water evaporation
Unfortunately I don't have the 4th one :/
