Question

You have isolated one of the genes for producing one of the blood-clotting proteins needed by some hemophiliacs. Briefly describe how you could create bacteria that would produce this protein

Answer 1

Isolate the plasmids of the bacterium, and use the same restriction endonuclease you have used to cut the gene for blood clotting proteins, to cut open the plasmid. Then use a ligase enzyme to join the genes with the open plasmid, to create a reccombinant plasmid. The plasmids are then taken up by the bacterium. The bacteria can multiply in a culture containing nutrients, so more genetically modified bacteria with the blood clotting genes, are produced. Or the bacteria can grow in a fermenter to produce commercial quantities of the protein