Question

In the B-galactosidase assay, it is important to measure the density of E. coli cells because Select one:

Answer 1

Answer:

Option C, E. coli densities vary among cultures and with time, which will affect estimates of b-galactosidase production rates.

Explanation:

The activity of β-galactosidase  depends on the optical density or the intensity of the color.

In regards to color, yellow color intensity signifies the qualitative parameter of the β-galactosidase activity while the readings obtained through colorimeter is quantitative parameter of the β-galactosidase activity. In the presence of lactose , the gene responsible for producing β-galactosidase in E. coli is either induced or switched off.

Hence , option D  is correct