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stich3 [128]
4 years ago
8

Why are two different restriction enzymes used to cut the puc19 plasmid and the lux gene DNA? What would have happened if only t

he HinD III enzyme was used?
Biology
1 answer:
snow_tiger [21]4 years ago
7 0
This is because there might not be one restriction site bordering the gene to be cloned and the identical restriction site in the plasmid. Since these two restriction enzymes make compatible sticky ends, the insert has a chance of combining with the plasmid. To ensure efficient digestion, the two recognition sites should be more than 10 base pairs apart. If one of the enzymes is a poor cutter or if the sites are separated 10 base pairs or less, the digestions <span>should be performed sequentially</span>
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Answer:

Key points

Carbon is an essential element in the bodies of living organisms. It is also economically important to modern humans, in the form of fossil fuels.

Carbon dioxide—\text {CO}_2CO

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Slow geological processes, including the formation of sedimentary rock and fossil fuels, contribute to the carbon cycle over long timescales.

Some human activities, such as burning of fossil fuels and deforestation, increase atmospheric \text{CO}_2CO

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start text, C, O, end text, start subscript, 2, end subscript and affect Earth's climate and oceans.

Carbon: building block and fuel source

About 18% of your body consists of carbon atoms, by mass, and those carbon atoms are pretty key to your existence!^1

1

start superscript, 1, end superscript Without carbon, you wouldn't have the plasma membranes of your cells, the sugar molecules you use for fuel, or even the \text{DNA}DNAstart text, D, N, A, end text that carries instructions to build and run your body.

Carbon is part of our bodies, but it's also part of our modern-day industries. Carbon compounds from long-ago plants and algae make up the fossil fuels, such as coal and natural gas, that we use today as energy sources. When these fossil fuels are burned, carbon dioxide—\text{CO}_2CO

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start text, C, O, end text, start subscript, 2, end subscript levels affects Earth's climate and is a major environmental concern worldwide.

Let's take a look at the carbon cycle and see how atmospheric \text{CO}_2CO

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start text, C, O, end text, start subscript, 2, end subscript and carbon use by living organisms fit into the bigger picture of carbon cycling.

The carbon cycle

The carbon cycle is most easily studied as two interconnected subcycles:

One dealing with rapid carbon exchange among living organisms

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Although we will look at them separately, it's important to realize these cycles are linked. For instance, the same pools of atmospheric and oceanic \text{CO}_2CO

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3

−

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