1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
stich3 [128]
3 years ago
8

Why are two different restriction enzymes used to cut the puc19 plasmid and the lux gene DNA? What would have happened if only t

he HinD III enzyme was used?
Biology
1 answer:
snow_tiger [21]3 years ago
7 0
This is because there might not be one restriction site bordering the gene to be cloned and the identical restriction site in the plasmid. Since these two restriction enzymes make compatible sticky ends, the insert has a chance of combining with the plasmid. To ensure efficient digestion, the two recognition sites should be more than 10 base pairs apart. If one of the enzymes is a poor cutter or if the sites are separated 10 base pairs or less, the digestions <span>should be performed sequentially</span>
You might be interested in
What is not a feature of rbc morphology?
kotegsom [21]

The given question is incomplete as options are not provided, however the options are as follows:

A. a large surface area-to-volume ratio

B. ability to form stacks known as rouleaux

C. a convex shape allowing for more efficient transport of oxygen

D. ability to bend and flex when entering capillaries

Answer:

The correct answer is - option C.

Explanation:

RBC cells are biconcave in the shape it is form shape of dub bell in general. These cells are small, round. The biconcave shape of the blood cells helps in the flow of the blood easily and these cells have no nucleus like other cells to have high amount of hemoglobin that is a pigment that carry oxygen and other gases for easy gaseous exchange.

Due to their flexible biconcave shape they can bend and flex accordingly during entering capillaries. The shape of the RBC helps in making stacks and large surface to area ratio.

Thus, the correct answer is - option C.

8 0
3 years ago
While performing an experiment at home, Emma adds lemon juice to a soap solution. How will this affect the pH of the soap soluti
kirza4 [7]
The pH will decrease because lemon juice is very acidic meaning it would bring the pH level down to a more acidic value.
4 0
3 years ago
In the skeletal system, which are the two main tissues responsible for structural support in the body?
attashe74 [19]
Bones and cartilages <span />
5 0
3 years ago
Which of the following is true about the cells of an animal’s body?
azamat

Answer:

D

Explanation:

5 0
3 years ago
What is the smallest unit in biology ?​
erma4kov [3.2K]

Answer:

A cell is the smallest unit of a living thing.

6 0
2 years ago
Read 2 more answers
Other questions:
  • What empirical evidence could most effectively and directly be used to convince law makers to remove a dam that is blocking a ri
    7·2 answers
  • Mutualism in a sentence
    15·1 answer
  • Hey do u guys know what are some good science fair topics
    9·1 answer
  • The ability to change body position and direction quickly and efficiently defines what skill
    15·1 answer
  • Which of the following is an example of how culture can help humans survive?
    10·1 answer
  • NEED THEM ASAP
    12·1 answer
  • Which of the following examples shows the influence coal has had on human activity?
    6·1 answer
  • What is occuring when the sound gets louder ? Check all that apply
    10·1 answer
  • The point of a reprimand should be to _______ an animal.
    9·1 answer
  • Which of the following is NOT one of the key characteristics of a well-designed test?
    5·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!