Answer:
The correct answer is -
a. Protonation of the substrate.
b. Deprotonation of the amino acids in the enzyme active site.
c. Change in tertiary structure in extreme conditions.
Explanation:
During an enzyme assay, suboptimal pH is changed and affect the Vmax to decrease and this could take place due to various reasons such as-
A) protonation of the substrate - Upon protonating or adding a proton or hydrogen cation to a substrate atom, the mass and the charge of the species each increase by one unit which make a conjugate acid.
B) Deprotonation of the amino acids in the enzyme’s active site -Changing the pH will affect the charges on the amino acid molecules.
C) Denaturation of the protein’s tertiary structure- Tertiary structure of the protein is basically a backbone of peptide chain with one or more secondary domains of proteins that increasing the pH or decreasing the pH get denature.
Thus, the correct answer is : option A, B, C.
Answer:
A) Surface-active agents, or surfactants, decrease surface tension among molecules of a liquid.
B) Phenolics exert antimicrobial activity by injuring lipid-containing plasma membranes, which causes the cellular components to leak out of the cell.
C) Alcohols exert their action by denaturing proteins and dissolving lipids.
Explanation:
Answer:
Temporary deafness is often the result of buildup within the ear canals.
Explanation:
