300 BC by Greek philosophers
Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
<span>the function of the mucus blanket in the lungs is to filter and moisten the air.</span>
Answer:
The 3 major observations were:
1. More individuals are produced each generation that can survive.
2. Phenotypic variation exists among individuals and the variation is heritable (through parents' genes).
3. Those individuals with heritable traits better suited to the environment will survive.
Explanation:
Charles Darwin was a British naturalist who proposed the theory of known to everyone as 'The Theory of Evolution' through natural selection.
Overuse of antibiotics is causing this resistance, we can let our bodies fight off bacterial infections naturally, stop the overuse of antibacterial soaps and cleansers, let our bodies build up resistance to these bacterias naturally through natural exposure.
