It has been challenging to develop methods for outer-membrane(OM) isolation from <em>Leptospira</em> species and other spirochetes due to their distinctive design and a lack of sensitive cytoplasmic membrane indicators.
Spirochetes have an OM and a cytoplasmic membrane, which are divided by a periplasmic space, just like enteric gram-negative bacteria.The leptospiral OM contains a significant amount of LPS, and the leptospiral surface is dominated by its polysaccharides.
LipL32, LipL36, and LipL41 are three lipoproteins from the leptospiral OM.
Since LipL32 is an immunodominant antigen during human leptospirosis, it is recognized to be the most prominent protein in the leptospiral protein profile. OmpL1 and LipL41 together offer synergistic immunoprotection. LipL41 is a surface-exposed lipoprotein.
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Answer:
50% G+C will have a higher Tm
Explanation:
The Temperature of Melting (Tm) refers to the temperature at which 50% of double-stranded DNA (dsDNA) is changed to single-standard DNA (ssDNA). In the double helix of DNA, Adenine bases always pair with Thymine bases through two hydrogen bonds, whereas Guanine bases always pair with Cytosine bases through three hydrogen bonds. In consequence, a DNA molecule containing a higher GC content is more stable than another DNA molecule containing a lower GC content. The Tm can be calculated as follows = 2 °C(A + T) + 4 °C(G + C) = °C Tm (this equation is useful for oligonucleotides of 14 to 20 base-length).
D an ecological footprint
Answer:
C. causes cell lysis
Explanation:
The slow release of the bacteriophage progeny from a bacterial host cell causes bacterial cell lysis and further kills the host cell which is the bacteria, this results to the liberation of progeny viruses which then infect new bacterial cells.
Only a type of bacteriophage called the filamentous bacteriophage reproduces without killing the bacterial host cell. The filamentous phages infect majorly gram negative bacteria.
Answer:
True
Explanation:
We are learning about this in science this week and im pretty sure this is true.
