You are given an experimental problem involving control of a geneʹs expression in the embryo of a particular species. One of you
r first questions is whether the geneʹs expression is controlled at the level of transcription or translation. Which of the following might best give you an answer?
a. You explore whether there has been alternative splicing by examining amino acid
sequences of very similar proteins.
b.You measure the quantity of the appropriate pre-mRNA in various cell types and
find they are all the same.
c. You assess the position and sequence of the promoter and enhancer for this gene.
d. An analysis of amino acid production by the cell shows you that there is an increase at
this stage of embryonic life.
e. You use an antibiotic known to prevent translation.
b.You measure the quantity of the appropriate pre-mRNA in various cell types and find they are all the same.
Explanation:
You are given an experimental problem involving control of a geneʹs expression in the embryo of a particular species. One of your first questions is whether the geneʹs expression is controlled at the level of transcription or translation is you measure the quantity of the appropriate pre-mRNA in various cell types and find they are all the same.
