The answer is C, I hope this helped you:)
Pure culture can be prepared from a mixture of cultures by using the streak plate method. This enables the bacterial cells to be diluted to obtain a pure culture after many-folds dilution.
Pure culture is the one in which only one species, strain or clone of bacteria are grown. The pure culture enables to scientists to study in detail about its anatomy, lifestyle and potential uses or harms, without the interference of any other microbe.
Streak-plate method is the most commonly used technique in microbiology labs to isolate a single type of bacteria. It is performed by creating the smears of bacteria in zig-zag motion to dilute its concentration.
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squeeze the fingers of the glove because of imcrease pressure
Frogs do have lungs but they are small and rarely used, frogs mostly take in moisture through the skin in process called cutaneous gas exchange.
