Confirm that the sample has both helicase and primase activities, but not the ability to synthesize DNA
- The replisome is a multiprotein-RNA complex that is required for the replication of DNA. The replisome consists of different proteins which include:
1. Helicase enzymes that unwind and separate the DNA strand
2. Replication factor C (RFC), a DNA-dependent ATP that acts as an activator of DNA polymerase.
3. Proliferating cell nuclear antigen (PCNA)
4. DNA gyrase/topoisomerase that cuts and reseals DNA strands, which is essential for DNA synthesis.
5. Primase enzyme that synthesizes short RNA fragments called primers.
6. DNA polymerase III, an enzyme that catalyzes the polymerization of deoxyribonucleotides into a DNA strand.
7. DNA ligases enzymes that form phosphodiester bonds at a single-strand break in DNA
- A primosome is a multiprotein complex responsible for creating RNA primers on single-stranded DNA (ssDNA) during the replication process.
- The primosome is composed of different primase and helicase enzymes. In<em> Escherichia coli</em>, it consists of PriA helicase, PriB, PriC, DnaB helicase, DnaG (encoded by the dnaG gene), DnaC and DnaT primase.
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Answer:
C. CH4 + 202 – CO2 + 2H20
Explanation:
CH4+O2-CO2+H20
The above equation represents combustion of methane to produce carbon-dioxide and water.
One carbon atom is present on the reactant side as well as on the product side. Four hydrogen atoms are present on the reactant side and two hydrogen atoms on product side. In order to balance this equation, we would multiply water molecule by two. Now there are four atoms of oxygen in the product side but only two on reactant side, so we will multiply oxygen molecule by two on the reactant side. Now the equation is balanced.
The balanced chemical equation is:
CH4+202-CO2+2H2O
This equation obeys the law of conservation of mass as the mass of the reactants is same as that of the products.
Answer:
c. A at the top of the saturated zone.
When assessing the heart rate of a 6-month-old infant, you should palpate the brachial or femoral pulse
Explanation:
Cold springs can affect the reproduction of rabbits as the rabbit's main priority is to survive before adding more to its family as they would already be struggling to keep itself warm. Yet alone a newborn rabbit which doesn't have any fur yet.
Also, cold springs could affect the amount of food that grows so the competition for food is more harsh so more rabbits are likely to die of starvation.
Though I'm not sure what your temperature range of a cold spring is so I might be giving extreme answers.
