1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Vadim26 [7]
3 years ago
14

You are performing a biochemical purification of enzymes involved in DNA replication. You have purified the replisome. You wish

to purify the primosome. You perform further separation techniques on your purified replisome. How will you test to determine that you have purified the primosome
Biology
1 answer:
algol [13]3 years ago
6 0

Confirm that the sample has both helicase and primase activities, but not the ability to synthesize DNA

  • The replisome is a multiprotein-RNA complex that is required for the replication of DNA. The replisome consists of different proteins which include:

            1. Helicase enzymes that unwind and separate the DNA strand

            2. Replication factor C (RFC), a DNA-dependent ATP that acts as an activator of DNA polymerase.

           3. Proliferating cell nuclear antigen (PCNA)

           4. DNA gyrase/topoisomerase that cuts and reseals DNA strands, which is essential for DNA synthesis.

           5. Primase enzyme that synthesizes short RNA fragments called primers.

          6. DNA polymerase III, an enzyme that catalyzes the polymerization of deoxyribonucleotides into a DNA strand.

           7. DNA ligases enzymes that form phosphodiester bonds at a single-strand break in DNA

  • A primosome is a multiprotein complex responsible for creating RNA primers on single-stranded DNA (ssDNA) during the replication process.
  • The primosome is composed of different primase and helicase enzymes. In<em> Escherichia coli</em>, it consists of PriA helicase, PriB, PriC, DnaB helicase, DnaG (encoded by the dnaG gene), DnaC and DnaT primase.

Learn more in:

brainly.com/question/14674568?referrer=searchResults

You might be interested in
Which of these most accurately describes the role of certain bacteria living in and around plant roots in the soil? A) They aera
algol13

Answer:

The answer is D

Explanation:

6 0
3 years ago
Read 2 more answers
How does an enzyme change the activation energy for a reaction in a cell?
k0ka [10]

Answer:

The correct answer is option c. "Enzyme lowers the activation energy".

Explanation:

The activation energy is defined as the amount of energy needed for a reaction to take place. Enzymes catalyze reactions by lowering the activation energy, which speeds up the reaction and increases its rate. Enzymes put a substrate in its active site, which facilitates the reaction and makes more likely that it collides with its subsequent substrate.

8 0
2 years ago
Cell Type Dichotomous Key
cupoosta [38]

Answer:

A animal 2b has no cell wall

8 0
3 years ago
A 12 pound shot put and a small metal BB are dropped from a tall tower, inside a vacuum chamber,
hichkok12 [17]

Given what we know, we should observe the objects falling at the same speed in both the tower and vacuum scenarios, but not in the water.

<h3>What affects the speeds at which these objects fall?</h3>
  • The falling speeds in both air and a vacuum will be the same for both objects.
  • This is because falling speed is determined by gravity and is independent of the mass of the objects falling.
  • The same will occur in water if and only if the densities of the two objects are equal.

Therefore, given that we can safely assume that the densities of the two objects are different from one another, we can confirm that while the objects will fall at the same speed in air and in a vacuum, this will not be the case in the deep pool.

To learn more about gravity visit:

brainly.com/question/4783082?referrer=searchResults

4 0
2 years ago
A missense mutation causes premature chain (protein) termination. A missense mutation causes premature chain (protein) terminati
timurjin [86]

Answer: The statement is false

Explanation:

NONSENSE mutation, NOT MISSENSE mutation causes premature chain (protein) termination.

Missense mutation is simply a kind of point mutation where a single nucleotide is changed to cause substitution of a different amino acid, thus resulting in a non-functional protein.

So, non-functional protein is caused by missense mutation making the statement false

6 0
3 years ago
Other questions:
  • You are doing a mark-recapture experiment to determine the population size of the MendAliens living on an island in my back yard
    14·1 answer
  • Many schools require applicants to take a test, such as the SAT or the ACT, as part of the admissions process. These tests deter
    15·2 answers
  • What do you think these moth wings mimic? Why do you think this kind of mimicry can protect the moth?
    6·1 answer
  • As the individual body size of an organism increases, the population density for this organism in a given habitat tends to
    13·1 answer
  • 1. By examining the fin of a primitive fish, scientists
    12·1 answer
  • Consider that a species of salmon lays 20,000 eggs per pair when it spawns and dies. At the end of five years, an average of one
    8·1 answer
  • Which statement best illustrates a biotic or an abiotic factor that is often found in a city park?
    6·1 answer
  • Which of the following terms refer to the total number of different species, including humans, on Earth or in an area? (Choose a
    15·1 answer
  • Skin color, fur color, and height are examples of which inheritance pattern?
    10·1 answer
  • Which of the below features was not used to identify the early Homo distinction from Australopithecines
    5·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!