Question

like other enzymes, carbonic anhydrase is affected by the pH of the solution surrounding it. describe the steps of an investigation to identify the ideal pH for the enzyme.

Answer 1

Answer:

Carbon dioxide is a significant metabolite for each living creature. It is a soluble compound that makes it simple to diffuse all through the cell. In contrast to carbon dioxide, bicarbonate needed to be transported. The response of CO2 and water as reactants to produce hydrogen and bicarbonate in the solution. This significant reaction is effortlessly kept up with the activity of carbonic anhydrase. The transformation of bicarbonate into CO2 and the other way around encourages bicarbonates transport into the cell just as trapping of CO2 in the cell.

This response is gradually continuing in physiological pH, so life forms produce enzyme as an enhancement of reaction speed. Contingent upon what is required, the active site of this catalyst can exist in two structures a high pH structure that is active in the hydration of CO2 and a low pH that is active in the dehydration of bicarbonate. For the best result, the temperature of equilibrium is significant.

Since the ideal temperature for the enzyme's response is in the scope of 35 to 40 degrees Celsius, the best temperature worth would be 37 degrees. In this manner, the arrangement for an examination would be taking off 5 examples warmed to correct degrees in the scope of 35 to 40, with sufficient measure of catalyst and watching the velocity of response and how quick will item shows up.