In some cats the gene for tail length shows incomplete dominance. Cats with long tails are homozygous dominant (TT) and cats wit
h no tails are homozygous recessive (tt). Heterozygous cats have short tails (Tt). For each of the following construct a punnett square and give phenotypic and genotype ratios of the offspring. a) a long tail cat and a cat with no tail b) a long tail cat and a short tail cat c) a short tail cat and a cat with no tail d) two short tail cats.
The Golgi apparatus is located in the cells. It is an extra combination of organelle similar to the ER. This was named following Camillo Golgi. (Notice that his last name is Golgi)
Cas9-sgRNA cannot align with targeted DNA sequence
Explanation:
Without a PAM sequence, the CRISPR-sgRNA (small guide RNA) complex cannot align with the targeted DNA sequence. Therefore, you cannot perform experiments (e.g. gene editing) using CRISPR-Cas9.