For staining flagella of bacteria use actively motile organisms 20 to 24 hours old, allow to diffuse in sterile water 20 to 30 minutes, transfer droplets of the suspension to clean slides and let evaporate without spreading. Then treat 2 to 4 minutes with the following mordant: tannic acid 10 or 20%, 50 cc.; ferric chloride 5%, 10 to 15 cc.; carbol fuchsin (Ziehl-Nielson), 5 cc.; hydrogen peroxide 3%, 6 to 8 cc. Wash and stain 2 to 3 minutes with a mixture of basic fuchsin, saturated alcoholic, 10 cc.; anilin oil (1 part) and 95% alcohol (3 parts) mixed, 5 cc.; distilled water, 30 cc.; acetic acid, 4%, 1 cc. Wash thoroly with water.
Answer:
Cell membrane is considered as boundary of the cell.
Explanation:
Cell membrane is the boundary of the cell which is selectively permeable that allows the micro-nutrients, water and gases like oxygen and carbondioxide etc through the process of diffusion. When the water goes inside due to diffusion it is the indicator that there is less quantity of water present inside the cell as compared to the external environment, this phenomena is called hypertonic.
Answer:
O Testable question are answered by looking up the answer ina book.
Answer:
photosynthesis is occuring and bubbles of carbon dioxide gas being released by the leaf into the surrounding area
Explanation:
Left ventricle pump blood to the whole body !